Fundamentals of LC-MS Video Training Course
4 Video Training Sessions, with Tutor Support
Fundamentals of LC-MS Video Course
This course has been designed for anyone who is either new to LC-MS, or who wishes to expand their current knowledge and understanding about the technique as a whole.
All aspects of the technology and system are covered - from an overview of a typical HPLC system and important considerations when combining this separation technique with detection by mass spectrometry, all the way through the various atmospheric ionisation processes and the different mass analysers that are available. The main focus is on fundamental concepts but important practical aspects and some basic troubleshooting will also be introduced.
During the 4 sessions we will discuss each phase of LC-MS analysis in detail. We will start by looking at the separation technique of liquid chromatography, including important components of a typical HPLC system and how they work together to drive the chromatography. There will also be an introductory overview of the components included within the mass spectrometer. In session 1 we will also cover the 3 main types of atmospheric ionisation, what is happening in the ion source and how to optimise the production of gas phase ions.
We will then go on to discuss the various different mass analysers that are available, concentrating on practical application and specific experimental designs. This will also cover elements such as ion fragmentation and MS-MS analysis.
Once the fundamental concepts of LC-MS have been covered we can then start to consider other important considerations regarding the HPLC component of this analysis technique. Looking at the effects that mobile phases and different buffers or additives can have on the quality of data produced. This will lead us to discuss the most suitable solvents for the different atmospheric ionisation techniques, as well as their effect on the mode of mass spectrometry used. As we go further through the course we will introduce the vacuum system associated with mass spectrometry and include some basic maintenance requirements for the various pumping systems
To complete this introduction to LC-MS there will be an overview of MS data interpretation strategies, concentrating primarily on small molecule identification and structural elucidation. We will then conclude with the important parameters that should be assessed when working to develop successful LC-MS methods.
Each session will be accompanied by supporting modules from CHROMacademy and a selection of short assessments. We will be using some of the many animations within CHROMacademy to help demonstrate the principles and processes being discussed. All four sessions should be attended in order to gain the level of understanding required to complete the Final Assessment. This is a mandatory element that you must complete to gain your certificate of course completion.
Course Delivery & Duration
Our CHROMacademy tutors will deliver four video training sessions over a four week period with all sessions made available on-demand after their release until the course end date.
It's important you register before the course start date! To gain a certificate of completion you must complete a final assessment before the course end date - this is typically seven weeks after we release session one.
Don't worry if you miss a session you can always catch up at anytime within the seven weeks period, however we strongly suggest that you keep up to date with the sessions as they are broadcast.
Who should take this course