UV-Visible Spectroscopy

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UV-Visible Spectroscopy

A UV/visible spectrophotometer is a device used to measure the transmittance or absorbance of a sample as a function of the wavelength of the electromagnetic spectrum. This module covers the fundamental scientific principles behind UV-visible spectroscopy, the principles of the instrumentation, and practical tips for optimizing the instrument parameters.

Topics include:

Bonding theory for UV-visible spectroscopy
The electromagnetic spectrum
Origin of UV-visible spectra
Transmittance and absorbance
Selection rules and intensity
UV-visible chromophores
UV-visible absorption maxima of chromophores
UV-visible definitions
UV-visible instrumentation
Optical arrangements of UV-visible spectrophotometers
UV-visible instrument configuration
Key UV-visible instrument parameters: spectral resolution and selectivity
Accuracy and precision
Linear dynamic range, calibration, and drift
UV-visible sample handling and measurement
Choice of solvent, and effect of solvent, concentration, pH, and temperature on UV-visible spectroscopy
Troubleshooting UV-visible spectroscopy

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