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Hydrophobic Interaction Chromatography of mAbs and ADCs – Not Just Another Way of Performing Reversed Phase - Like Separations
Live Webcast - Thursday 21st & 22nd June 2018

We have covered a wide range of topics in our Biochromatography webcasts on protein characterization during the past few months. In this latest webcast, we are introducing a different chromatographic technique: hydrophobic interaction chromatography. Although there are similarities to reversed phase chromatography with proteins eluting in order of increasing hydrophobicity, the similarities end there! Learn more by listening in.

Hydrophobic Interaction Chromatography of mAbs and ADCs »

   
 
Choosing the Right Column for Intact Protein Characterization

This webcast will investigate some of the parameters you need to take into consideration when choosing the most appropriate chromatography column to get the best out of your instrumentation and provide the optimum resolution for your protein characterization.

Choosing the Right Column for Intact Protein Characterization »

   
 
UV or MS, Derivatized or Not? Choosing and Optimizing a Workflow for Amino Acid Analysis in Spent Media

This webcast will discuss workflow options for spent media analysis, focusing on LC/UV and LC/MS methods.  Guidance will be given for which workflow to choose depending on your needs and priorities.  Additionally, tips to get the best data from each approach will be discussed.

UV or MS, Derivatized or Not? Choosing and Optimizing a Workflow for Amino Acid Analysis in Spent Media »

   
Method Development for Ion Exchange Using Dynamic Buffer Generation

This webcast will focus on understanding the complexity of biotherapeutic protein charge variant analysis and how optimization of weak cation exchange chromatography is a necessity.

Method Development for Ion Exchange Using Dynamic Buffer Generation »

   
Choosing the Correct Column and Mobile Phase for LC-MS Peptide Mapping

This webcast will focus on essential parameters for successful peptide mapping by LC/MS. Obtaining complete sequence coverage as well as separation and detection of low-level post-translational modifications is a challenging endeavor.

Choosing the Correct Column and Mobile Phase for LC-MS Peptide Mapping »

 
Choosing the Correct Column & Mobile Phase for LC MS Characterization of Biologics

This webcast will focus on the parameters which are essential for the development of a successful RPLC-MS method for the characterization of a monoclonal antibody. 

Choosing the Correct Column & Mobile Phase for LC MS Characterization of Biologics »

 
Techniques Employed in Biopharmaceutical Analysis Making the Right Choices in SEC Method Development

This webcast will focus on the parameters which are essential for the development of robust size exclusion methods; selecting the correct column pore size for your molecules, how to design optimum mobile phases for resolution, and the manipulation of parameters such as column dimensions and flow rate to allow for faster analyses...

Making the Right Choices in SEC Method Development »

 
Techniques Employed in Biopharmaceutical Analysis Advances in Full Characterization of Protein Biopharmaceutical Post Translational Modifications

Post translational modification (PTMs) which are most characteristic in protein biopharmaceuticals include glycosylation (which comprises galactosylation, fucosylation, high mannose derivatives and sialylation), oxidation, phosphorylation, sulfation, lipidation, disulfide bond formation and deamidation, as well as...

Advances in Full Characterization of Protein Biopharmaceutical Post Translational Modifications »

 
Techniques Employed in Biopharmaceutical Analysis Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC

This webcast shall interrogate the critical parameters required to succesfully implement and optimize reversed phase analysis of biopharmaceuticals. We will also discuss the popularity behind glycosylated biopharmaceuticals, where heterogeneity arises in these molecules, and how glycans can be effectively liberated, characterized, and analyzed using HILIC.

Techniques Employed in Biopharmaceutical Analysis - Part I Reversed Phase and HILIC »

 
Techniques Employed in Biopharmaceutical Analysis Techniques Employed in Biopharmaceutical Analysis - Part II Ion Exchange and SEC

Ion exchange (IEX) and size exclusion chromatography (SEC) provide much more information than merely charge variance and aggregation analysis. In this event we will discuss how IEX and SEC are typically employed in biopharmaceutical characterization and quantification, highlight strategies for developing more robust methods, consider the effect of temperature and pressure on SEC separations, and the role of UHPLC. Finally an introduction to hydrophobic interaction chromatography (HIC) will also be given.

Techniques Employed in Biopharmaceutical Analysis - Part II Ion Exchange and SEC »

 
HPLC Techniques in Biopharmaceutical Analysis HPLC Techniques in Biopharmaceutical Analysis

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This Essential Guide webcast will provide details of why differing modes of chromatography are required in the research, preparation, and analysis of biopharmaceuticals and when they would be typically used.

HPLC Techniques in Biopharmaceutical Analysis »

 
Characterization of Protein Biopharmaceuticals Characterization of Protein Biopharmaceuticals

Protein biopharmaceuticals, such as monoclonal antibodies and recombinant proteins, are currently in widespread use for treatment of various life-threatening diseases including cancer, autoimmune diseases, diabetes, anemia, etc. Protein therapeutics have a complexity far exceeding that of small molecule drugs, hence, unraveling this complexity represents an analytical challenge. This webcast will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

Characterization of Protein Biopharmaceuticals »

 
The Analysis of Post Translational Modifications Using LC-MS/MS The Analysis of Post Translational Modifications Using LC-MS/MS

This webcast will explain the principles behind the analysis of a number of key post-translational modifications (PTMs) using LC-MS/MS techniques. The use of bottom-up proteomics and different MS scanning techniques to characterize PTMs such as, phosphorylation, acetylation, ubiquitination and SUMOylation will be covered.

The Analysis of Post Translational Modifications Using LC-MS/MS »

 
Reversed Phase HPLC for the Analysis of Biomolecules Reversed Phase HPLC for the Analysis of Biomolecules.

Commonly reversed phase (RP), hydrophilic interaction (HILIC), ion exchange (IEX), and size exclusion (SEC) chromatographic modes will be combined to allow full characterization of complex biomolecules. The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in their analysis.

Reversed Phase HPLC for the Analysis of Biomolecules »

 
HILIC, IEX, and SEC for the Analysis of Biomolecules HILIC, IEX, and SEC for the Analysis of Biomolecules

The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. This article will examine the use of HILIC, IEX, and SEC for their full characterization.

HILIC, IEX, and SEC for the Analysis of Biomolecules »

 
Quick Guide Biomolecules Selecting Columns and Initial Conditions for IEX and SEC

Biopharmaceutical compounds are genetically engineered from living cells and have molecular weights in the range 2,000-2,000,000 Da with 10-2,000 reactive groups and are often comprised of a mixture of closely related variants; therefore, there is a wider degree of variability (a degree of heterogeneity) in comparison with small molecule pharmaceuticals. 

Selecting Columns and Initial Conditions for IEX and SEC »

 

Insights into Optimizing Bioseparation Workflows for Critical Quality Attributes (CQA) Assessment - eBook


Key areas covered in this eBook include:

  • Optimizing a workflow for amino acid analysis of spent media
  • Choosing the right column for intact protein characterization
  • Expert insight on reversed-phase analysis of proteins
Download our Free eBook »
 


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