Troubleshooting Pump Issues
This learning path on troubleshooting HPLC pump issues covers flow inconsistencies, pressure fluctuations, leaks, and air bubbles. It includes diagnosing check valve failures, degassing solvents, seal maintenance, and piston wear. Practical strategies focus on system calibration, preventative maintenance, and optimizing pump performance for stable, reproducible chromatography.
1 Module 2 Quick Guides
3 Items
HPLC Solvent Pumping Systems
The aim of this unit is to describe the role of the solvent delivery system (pump) within high pressure liquid chromatography (HPLC). The operational principles of the most popular solvent delivery types are explained, as well as the function of the components and potential troubleshooting and maintenance issues. We will describe good practice in pump use, care and storage, and discuss the operating principle of alternative pump designs such as preparative and micro-flow pumping systems.
HPLC Troubleshooting Video Guide to High Pump Backpressure
High backpressure in the system results in the pump working under greater resistance which will lead to an increase in the need for maintenance. Learn how to locate the source of high backpressure in your HPLC system and resolve the problem.
