Troubleshooting Autosampler Issues
A learning path on troubleshooting HPLC autosampler issues covers injection errors, carryover, misalignment, and leakage. It includes diagnosing needle blockage, syringe wear, and vial positioning problems. Practical strategies focus on maintenance, cleaning, optimizing injection parameters, and ensuring precise, reproducible sample delivery for accurate chromatography.
1 Module 1 Webcast 3 Quick Guides
5 Items
Troubleshooting Autosampler Issues
Mechanically complicated, autosamplers have the ability to create a variety of troubleshooting problems. By focusing on each part of the autosampler we will provide you with knowledge of where problems can arise - and how to reduce the likliehood of this through proper use and maintenance. We highlight the common autosampler related problems to ensure that you recognize them and their source quickly to reduce instrument down time.
Autosamplers
The aim of this module is to explain the purpose of injectors and autosamplers within the HPLC system. The principles behind which sample injection systems work will be described, as well as the individual components of an injection valve. The correct and incorrect ways to use loop injectors in manual injection mode will be highlighted, and we will discuss the major components and operating principles of a range of typical autosampler devices.
Autosampler Contamination
Contamination in any chromatographic method is a complex problem to troubleshoot. Extra peaks, often referred to as ghost peaks, in a blank or sample chromatogram can have a number of sources. This quick guide will focus on autosampler contamination, how to determine if the autosampler is the source, and how to remedy the problem.
Troubleshooting Chromatography Vials
This quick guide looks at the 7 most common problems we get asked about in relation to chromatography vials.
An Alternative Approach to Troubleshooting Variable Peak Height/Area in HPLC
The problem of variable peak height/area is a common and complex one, with many potential causes. We have constructed an Ishikawa diagram to look at the possible causes of variable peak height/area and to help prioritize the approach to fixing the problem.