SPE Method Development
This learning path on SPE method development covers sorbent selection, sample preparation, and optimization of extraction steps (conditioning, loading, washing, and elution). It includes choosing appropriate solvents, pH adjustments, and flow rates. Practical strategies focus on maximizing recovery, minimizing matrix effects, and ensuring reproducibility for accurate analytical results.
2 Modules 1 Webcast 1 Quick Guide
4 Items
SPE Mechanisms
The purpose of this module is to highlight the various steps in the protocol for various modes (sorbents) for conventional solid phase extraction procedures including: non-polar SPE, polar SPE, cation exchange SPE, anion exchange SPE, and mixed-mode SPE. For each mode the various portocol steps are examined in terms of the solvents and solutions used, sorbents used, solvent additives, pH, and ionic strength manipulation.
SPE Method Development
This module fully investigates the various aspects of practical SPE, as well as describing the basic principles and stages of method development in SPE.
SPE Method Development Interactive Learning Tool
We look at a simple and structured approach to developing a reversed phase solid phase extraction (SPE) method. We’ve provided an interactive learning tool to allow you to evaluate different sets of conditions and see what effect they have on the resulting chromatogram.
Top Tips to Improve Reproducibility and Sensitivity in Solid Phase Extraction
The material provided in this webcast will give you further insight into the fundamentals of SPE as well as the process of method development to best give a robust and sensitive method. We will give tips for improving both SPE reproducibility and sensitivity.