HPLC Extra Peaks and Contamination

HPLC Extra Peaks and Contamination

The problem of extra peaks and contamination is one of the most common problems the CHROMacademy team get asked about. Therefore, this concise training session will give you quick, practical tips to get you on the path to resolving this complex problem when it arises.

Troubleshooting Extra Peaks in HPLC

In this module we’re going to take a look at a very common problem in HPLC – extra peaks and contamination. We’ll look at the main types of contamination, the potential sources, defining the problem and main types of investigational re-analysis. The module is supplemented by real life case study workshops, in which you have a virtual HPLC that allows you to select what additional investigations you’d like to perform.

HPLC Troubleshooting Strategy

This five step troubleshooting strategy will set you up for success when troubleshooting any HPLC problem.

The Rights and Wrongs of Mobile Phase Preparation

Mobile phase preparation - what could possibly go wrong? Let us walk you through a typical mobile phase preparation process highlighting how to avoid common pitfalls such as contamination from glassware or mixing organic and aqueous in the incorrect order. After watching this webcast there will be much more right than wrong with your mobile phase preparation.

The Rights and Wrongs of Mobile Phase Preparation Q&A

This Q&A session, based on the questions submitted in one of our most attended webcasts, will make you think about mobile phase preparation in a whole new way! Everything from how cleaning our glassware can impact analyses, to how to avoid or minimize contamination, what the best practices are for filtering mobile phases - and lots in between.

Autosampler Contamination

Contamination in any chromatographic method is a complex problem to troubleshoot. Extra peaks, often referred to as ghost peaks, in a blank or sample chromatogram can have a number of sources. This quick guide will focus on autosampler contamination, how to determine if the autosampler is the source, and how to remedy the problem.