HPLC Column Fundamentals
This learning path on HPLC column fundamentals covers stationary phase chemistry (C18, C8, phenyl, etc.), column dimensions (length, diameter, particle size), and selection for different applications. It includes maintenance practices like flushing, storage, and troubleshooting performance issues. Practical strategies focus on optimizing separation, longevity, and reproducibility in chromatographic analysis.
1 Module 1 Webcast 3 Quick Guides
5 Items
HPLC Column Chemistry
The aim of this module is to introduce silica as a major stationary phase support for reversed phase HPLC separations and for normal phase applications. The use of bonded phases in various chromatographic applications, and the influence of surface silica silanol groups on chromatographic separations is illustrated. Modern bonded phases that give an advantage to today’s chromatographer are introduced.
HPLC Column Dimensions
HPLC column dimensions will affect the efficiency sensitivity, and speed of an analysis. The primary column dimensions of particle size, length, and internal diameter will be discussed and their effect on chromatographic separations demonstrated, providing some practical insight into how to select the best column dimensions for a particular application.
How to Choose an HPLC Column
Here we share some great general hints and tips for HPLC column selection.
How to Select the Optimum HPLC Column
Here are the top tips to get column selection right every time! Selecting the correct HPLC column can greatly impact on chromatographic results and analysis speed. This webcast will allow you to understand how to select a stationary phase based on analyte chemistry, how particle morphology can be used to improve resolution, and how column dimensions can be altered to increase throughput.
HPLC Column Maintenance - Prevention is Better than Cure
Preventing problems before they occur is always preferable to troubleshooting complex problems. This quick guide will look at practical steps that can be taken to ensure columns are well maintained so that they can perform as required, including dedicating columns, using in-line filters, guard columns or disks, utilizing sample clean up, proper column storage, and column replacement.