Basic HPLC Instrumentation

Practical HPLC Video Bootcamp

Join us for a little over an hour on this step-by-step guide through setting up an HPLC instrument. Avoid common errors and pitfalls and understand every step of the process to get your instrument set up correctly every time, decrease down-time, and reduce the risk of re-analysis. 

Mobile Phase Considerations

The aim of this module is to explain the purpose of the mobile phase in HPLC. Topics include the best way to prepare mobile phase, potential problems caused by improper preparation and use of mobile phases, and the way that mobile phase composition effects retention and selectivity in reverse phase HPLC.

Autosamplers

The aim of this module is to explain the purpose of injectors and autosamplers within the HPLC system. The principles behind which sample injection systems work will be described, as well as the individual components of an injection valve. The correct and incorrect ways to use loop injectors in manual injection mode will be highlighted, and we will discuss the major components and operating principles of a range of typical autosampler devices.

HPLC Solvent Pumping Systems

The aim of this unit is to describe the role of the solvent delivery system (pump) within high pressure liquid chromatography (HPLC). The operational principles of the most popular solvent delivery types are explained, as well as the function of the components and potential troubleshooting and maintenance issues. We will describe good practice in pump use, care and storage, and discuss the operating principle of alternative pump designs such as preparative and micro-flow pumping systems.

HPLC Detectors

The aim of this module is to describe the purpose and general operating principles of detectors for HPLC. The general terms and concepts by which detector operation and performance are outlined. Specific operating principles of a range detectors for HPLC are covered, including UV-visible, refractive index, and fluorescence detectors.