LCMS Bootcamp

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Principles
Technique
Instrumentation
Troubleshooting
Method Development
Miscellaneous
Learning Paths

Technique
Troubleshooting
Method Development
Learning Paths

Principles
Technique
Instrumentation
Troubleshooting
Method Development
Learning Paths

Principles
Technique
Instrumentation
Troubleshooting
Method Development
Learning Paths

Principles
Technique
Learning Paths

Principles
Technique
Troubleshooting
Learning Paths

Principles
Technique
Instrumentation
Method Development
Miscellaneous
Learning Paths

Principles
Technique
Instrumentation
Learning Paths

Modules

Mobile Phase Considerations
HPLC Band Broadening
HPLC Introduction
Column Chemistry
HPLC Chromatographic Parameters
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Quick Guides

HPLC Mobile Phase Lifetimes
Buffer Choice for HPLC Separations
HPLC Filtration - Rocks, Boulders, and Sand
HPLC Extra Column Volumes
HPLC Column Dimensions
+ VIEW MORE GUIDES

Webcasts

20 Steps to a Reliable Mobile Phase - Q & A
CHROMtalks - Fundamentals and Principles of Extra Column Band Broadening in UHPLC: A Tutorial
The Rights and Wrongs of Mobile Phase Preparation Q&A
The Rights and Wrongs of Mobile Phase Preparation
20 Steps to a Reliable HPLC Mobile Phase
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Video Courses

Fundamentals of HPLC Video Training Course

Modules

Aqueous Normal Phase (ANP) Chromatography
HPLC Method Review and Workload Planning
Practical HPLC Video Bootcamp
Mixed-Mode HPLC
Ion Chromatography
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Quick Guides

HPLC Derivatization
HILIC Mechanism and Stationary Phase
HILIC Conditioning and Equilibration
How to Choose a Stationary Phase for HILIC
Ion Pair Alternatives
+ VIEW MORE GUIDES

Webcasts

CHROMtalks - Key Issues with Sample Preparation for Liquid Chromatography
System Suitability for HPLC
CHROMTalks HPLC Series
Hydrophilic Interaction Chromatography (HILIC) and Related Techniques
Making HILIC Work for You
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Modules

Practical HPLC Video Maintenance
UV-Visible Detectors
Introduction to HPLC Detectors
HPLC Detectors
HPLC Solvent Pumping Systems
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Quick Guides

HPLC Essentials - Switching Between Reversed and Normal Phase
HPLC Essentials - System Dwell Volume
Essential HPLC Protocols
5 Diode Array Detector Settings That Should be Optimized
Autosampler Contamination
+ VIEW MORE GUIDES

Webcasts

Leveraging Optimal Performance of Charged Aerosol Detection Through Optimization and Troubleshooting Techniques
What HPLC Operators Need to Know Part 1: Everything Needed to Understand Mobile Phase Pumping Systems
Everything You Need to Know About UV Detectors for HPLC
HPLC Detectors – What, Where, When, and How
Everything You Should Know About UV Detection for HPLC
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Modules

Troubleshooting Extra Peaks in HPLC

Quick Guides

HPLC Troubleshooting Strategy
HPLC Troubleshooting Guide to Peak Fronting and Poor Sensitivity
HPLC Troubleshooting – Retention Time and Baseline Issues
HPLC Troubleshooting - More Split Peaks
HPLC Troubleshooting Video - Peak Tailing
+ VIEW MORE GUIDES

Webcasts

HPLC Column Troubleshooting - Can We Fix It?
Troubleshooting HPLC Mobile Phase Issues - Q&A
CHROMtalks - The Top Seven Autosampler Problems and How to Avoid Them
CHROMtalks - Listen to Your LC Instrument - It Is Trying to Tell You Something!
CHROMtalks - Essentials of LC Troubleshooting - Some Problems Just Never Go Away
+ VIEW MORE WEBCASTS

Modules

Fast HPLC

Quick Guides

Speeding Up Your HPLC
You Need a Fancy System to Run Fast HPLC – Or Do You?
Three Quick Tips to Improve HPLC Method Development
How U is Your UHPLC?
Is the Art of HPLC Method Development Dead?
+ VIEW MORE GUIDES

Webcasts

CHROMtalks - (More) Steps Toward Easier Method Development for Two-Dimensional Liquid Chromatography
CHROMtalks - A Simple Approach to LC Method Development
CHROMtalks - Beyond the HPLC Column: Understanding Non-Column Factors That Influence Peak Widths and Shapes in High-Efficiency Separations
CHROMtalks - A Generic Methodology for Streamlined Performance Monitoring of a Wide Range of Reversed Phase UHPLC Columns Over Time
CHROMtalks - The Selectivity Landscape in Reversed-Phase LC—Using Selectivity Models to Find Columns that Are Similar, or Different
+ VIEW MORE WEBCASTS

Video Courses

HPLC Method Development Video Training Course

Modules

HPLC Interactive Tools

Modules

Introduction to GC Columns
GC Columns
GC Introduction
GC Chromatographic Parameters
GC Band Broadening
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Quick Guides

Packed or PLOT GC Columns for Gas Analysis?
GC Band Broadening - Longitudinal Diffusion
GC Chromatographic Parameters - Efficiency
GC Columns - Analyte/Stationary Phase Interactions
How Important is it to Know the Correct GC Column Length?
+ VIEW MORE GUIDES

Video Courses

Fundamentals of GC Video Training Course

Modules

GC Method Review
Introduction to System Suitability for Gas Chromatography
Internal Standards
Preparing Standards for GC
Practical GC Video Bootcamp
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Quick Guides

GC Headspace Vial Crimping
GC Derivatization
GC Sampling Techniques - Headspace
The Why, What, and How of Headspace GC
Optimizing GC Temperature Programming
+ VIEW MORE GUIDES

Webcasts

CHROMtalks - Current Issues with Sample Preparation for Gas Chromatography
CHROMTalks GC Series
GC Temperature Programming - 10 Things You Absolutely Need to Know
Everything You Should Know About Headspace GC
Understanding 2D Gas Chromatography
+ VIEW MORE WEBCASTS

Modules

Introduction to the Split/Splitless Inlet
Introduction to GC Gas Filters
Gas Cylinders
GC Oven Temperature
GC Ferrules
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Quick Guides

GC Autosampler Setup
Best Practice for GC Sample Introduction
5 Ways to Improve Your Split/Splitless Injection Technique
How to Optimize Key Variables in GC Analysis - Sample Introduction
Do We Need Glass Wool in a GC Inlet Liner?
+ VIEW MORE GUIDES

Webcasts

CHROMtalks - Optimizing Sample Introduction in GC through Focusing Techniques and Appropriate Inlet Selection
Principles of Operation, Maintenance, and Troubleshooting for Sulfur and Nitrogen Chemiluminescence Detectors with Gas Chromatography
CHROMtalks - GC Sample Introduction - The Achilles Heel of GC
CHROMtalks - Qualifying and Optimizing GC Instruments and Methods in High-Throughput Regulated Laboratories
CHROMtalks - Detecting It All - Keeping Your Response Consistent with Common GC Detectors
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Quick Guides

Practical GC Troubleshooting - Reduced Sensitivity
10 Common GC Mistakes
GC Troubleshooting Guide to Broad Peaks and Loss of MS Sensitivity
Practical GC Troubleshooting Video - Peak Tailing
Practical GC Troubleshooting Video - Peak Splitting
+ VIEW MORE GUIDES

Webcasts

CHROMtalks - Troubleshooting GC Sample Introduction
CHROMtalks - GC Troubleshooting in Five Simple Pictures
CHROMtalks - Hidden Errors and Mistakes that Make Your GC Results Look Better than They Really Are
GC Troubleshooting - Gas Supply and Inlet Issues
GC Troubleshooting in 20 Pictures - Part I
+ VIEW MORE WEBCASTS

Quick Guides

Real Life GC Column Selection
GC-MS Columns for GC Methods
How to Optimize Key Variables in GC Analysis - GC Columns and Detectors

Webcasts

CHROMtalks - Comprehensive Two-Dimensional Gas Chromatography: Tips on Method Optimization
CHROMtalks - Using Classical Discussions of Separation in Gas Chromatography to Make Our Methods More Efficient and Greener
CHROMtalks - GCxGC: It’s Not That Hard - Really!
CHROMtalks - GC Column Selection: The Three Most Important Parameters to Consider
Choosing the Right GC Column - The Most Important Choice You Will Ever Make
+ VIEW MORE WEBCASTS

Video Courses

GC Method Development Video Training Course

Modules

GC Interactive Tools

Modules

QuEChERS
SPE Overview
Molecular Properties
Primary Sample Preparation Techniques
Liquid/Liquid Extraction Techniques
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Quick Guides

Soxhlet Extraction
Vacuum or Positive Pressure: Which is Best for SPE Processing?
Sample Preparation - Microextraction: SDME
SPE - Column Conditioning
Modern SPE Sorbents
+ VIEW MORE GUIDES

Webcasts

CHROMTalks Sample Preparation Series
Everything You Need to Know About Filtration
Understanding the Mechanisms of Solid Phase Extraction
Choosing the Correct Sample Preparation Technique

Quick Guides

SPE - Troubleshooting Recovery Problems
Investigating SPE Recovery Problems
Troubleshooting Filtration
Troubleshooting SPE Cleanliness
Troubleshooting SPE Reproducibility Problems
+ VIEW MORE GUIDES

Webcasts

Troubleshooting Sample Preparation: Filtration, LLE, SLE, and SPE
Troubleshooting Sample Preparation

Modules

SPE Method Development

Quick Guides

SPE Method Development Interactive Learning Tool
SPE Sorbent Screening
Optimizing Sample Preparation - Filtration
SPE Mechanism Selection
SPE Procedure Optimization
+ VIEW MORE GUIDES

Webcasts

CHROMtalks - Elevating Analytical Excellence: Innovations in Method Development with Automated Sample Preparation (Part 2)
CHROMtalks - Elevating Analytical Excellence: Innovations in Method Development with Automated Sample Preparation (Part 1)
CHROMtalks - Sample Preparation Techniques Used in LC Method Development
Improved Speed of Extractables Studies Using Accelerated Solvent Extraction and LC-MS
Top Tips to Improve Reproducibility and Sensitivity in Solid Phase Extraction
+ VIEW MORE WEBCASTS

Modules

GC-MS Introduction

Webcasts

Understanding GC-MS Analysis

Video Courses

Fundamentals of GC-MS Video Training Course

Modules

GC-MS Ion Ratio Confirmation - Qualifier and Identifier Ion Techniques
Using Hydrogen as a Carrier Gas in GC-MS
GC-MS Library Searching
GC-MS Acquisition Modes
Practical GC-MS Video Bootcamp
+ VIEW MORE MODULES

Quick Guides

GC-MS Column Selection
GC-MS Ionization Quick Guide
Fundamentals of GC-MS Ionization Techniques

Webcasts

Understanding Electron Ionization for GC-MS
GC-MS Ionization Processes
What GC-MS Operators Need to Know
GC-MS/MS Analysis – What You Need to Know!

Modules

GC-MS Vacuum Systems
GC-MS Sample Introduction
Introduction to Mass Analyzers
Quadrupole Mass Analyzers
Time-of-Flight (ToF) Mass Analyzers
+ VIEW MORE MODULES

Quick Guides

GC-MS Solvent Delay
Vacuum Pump Maintenance
What Can a GC-MS Tune Report Tell You?

Quick Guides

GC-MS Troubleshooting - Sensitivity
GC-MS Contamination
GC-MS Leak Detection

Quick Guides

Do You Know What is in Your Beer?
Does Mass Spectrometry Need Chromatography?
Improving Sensitivity of Environmental GC-MS Analysis

Webcasts

CHROMtalks - Why and How to Develop A Non-targeted GC(MS) Screening Method
Translating GC and GC-MS Methods
The Secrets of Optimizing GC-MS Methods
Optimizing GC-MS and GC-MS/MS Analysis for Environmental Applications

Modules

Electrospray Ionization (ESI) Theory
Atmospheric Pressure Chemical Ionization (APCI) Theory
Atmospheric Pressure Photoionization (APPI) Theory
LC-MS Introduction

Video Courses

Fundamentals of LC-MS Video Training Course

Modules

Practical LC-MS Video Bootcamp
LC-MS Interpretation
Atmospheric Pressure Chemical Ionization (APCI) Technique
Atmospheric Pressure Photoionization (APPI) Technique
Electrospray Ionization (ESI) Technique
+ VIEW MORE MODULES

Quick Guides

LC-MS - Effect of Solvent Type and pH
10 Must Know Points to Understand Electrospray Ionization
New to LC-MS? 10 Practical Tips That Will Make a Real Difference
7 Must Know Points to Understand Atmospheric Pressure Chemical Ionization

Webcasts

CHROMTalks Mass Spectrometry Series
What LC-MS Operators Need to Know
Optimizing API Interfaces
Electrospray Ionization (ESI) for LC-MS

Modules

Time-of-Flight (ToF) Mass Analyzers
Electrospray Ionization Instrumentation
Introduction to Mass Analyzers
Vacuum Systems
Orbitrap Mass Analyzers
+ VIEW MORE MODULES

Quick Guides

Vacuum Pump Maintenance
Everything You Need to Know About LC-MS Tuning and Calibration

Webcasts

Fundamentals of Triple Quadrupole MS in Applied Markets
Understanding Mass Analyzers - Traps and ToFs
Quadrupole Analyzers and MS/MS Analysis

Quick Guides

ESI Signal Suppression
10 Ways to Break Your LC-MS
Controlling Na and K Adducts in LC-MS
10(ish) Ways to Prevent LC-MS Contamination
Avoiding LC-MS Signal Suppression
+ VIEW MORE GUIDES

Webcasts

Troubleshooting LC-MS

Modules

Electrospray Ionization eLab
Atmospheric Pressure Chemical Ionization eLab
Atmospheric Pressure Photoionization eLab
Flow Rates and Flow Splitting eLab
Solvents, Buffers, and Additives eLab
+ VIEW MORE MODULES

Quick Guides

LC-MS - Introduction to MS/MS Experiments
Does Mass Spectrometry Need Chromatography?
10 Tips For Great LC-ESI-MS Results
Quick LC-MS Method Optimization

Webcasts

Chromatographic Analysis of PFAS: Method Development and Optimization
The Challenges of PFAS Analysis
Optimizing LC-MS and LC-MS/MS
LC-MS Method Parameters - Critical Evaluation

Modules

Introduction to IR Spectroscopy

Webcasts

Introduction to Infrared Spectroscopy

Modules

Infrared Spectral Interpretation
UV-Visible Spectroscopy

Webcasts

Fundamentals of ICP-MS
Fundamentals of ICP-OES
Fundamentals of Atomic Absorption Spectroscopy

Modules

Fundamental Chemistry

Webcasts

Graduate to Analyst - What It Takes

Modules

Microbalance Weighing
Karl Fischer Titration
Glass Pipettes
Eliminating Error During Preparation of Samples
Automatic Pipettes
+ VIEW MORE MODULES

Quick Guides

10 Questions We've Been Asked About Balances

Webcasts

Weighing, Pipetting, and Volumetric Glassware – More than Just Basic Lab Skills
Tips and Tricks to Improve Essential Lab Practices: Weighing, Pipetting, and Volumetric Glassware
Coping With COVID-19: Remaining Productive and Safe in the Analytical Laboratory

Quick Guides

Troubleshooting Chromatography Vials
Top 10 Pipetting Mistakes

Modules

Biopharmaceuticals
Fundamentals of MS Proteomics Research
Introduction to Biopharmaceutical Analysis
mAbs - Introduction to Monoclonal Antibodies
Amino Acids, Peptides, and Proteins
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Quick Guides

The Fundamentals of HPLC Peptide Analysis
The Fundamentals of Glycan Analysis
Introduction to Amino Acid Analysis in Biopharmaceuticals

Webcasts

An Introduction to Biomolecules

Modules

Introduction to Ion Mobility Spectrometry (IMS)
Mass Spectrometry at the Glycan Level
Mass Spectrometry at the Peptide Level
Reversed Phase - Protein Level Analysis and Optimization
HILIC for Biomolecules
+ VIEW MORE MODULES

Quick Guides

Fundamentals of Biomolecules
First Steps in Characterizing Biotherapeutics
Reversed Phase HPLC for the Analysis of Biomolecules
HILIC, IEX, and SEC for the Analysis of Biomolecules
Hydrophobic Interaction Chromatography for Biopharmaceutical Analysis
+ VIEW MORE GUIDES

Webcasts

HPLC Analysis of Oligonucleotides
LC/MS MAM Analysis of Adeno-Associated Virus Particles
CHROMtalks - Tips and Tricks for Contemporary LC Separations of Biomolecules
In-depth Characterization of Biopharmaceuticals by Multidimensional LC-MS
High-throughput and High-resolution N-glycan Profiling Workflows Using InstantPC
+ VIEW MORE WEBCASTS

Modules

Mass Analyzers Used in Biomolecule Analysis

Webcasts

The Benefits of Dedicated HPLC Systems and Chromatography Columns for Analysis of Biomolecules

Quick Guides

Demystifying Reversed Phase Column Selection for Biomolecule Analysis
Selecting Columns and Initial Conditions for IEX and SEC Biomolecule Analysis
Developing SEC Methods for Biologics Analysis

Webcasts

Advanced Column Selection and Method Optimization for Biomolecular Purification and Characterization
Unlock Efficiency and Simplify Scale-Up with AdvanceBio Oligonucleotide Columns: From Analytical to Preparative Purification
CHROMtalks - The Use of Machine Learning to Automate Method Development for LC Separations of Biopharmaceuticals
Upgrading Your Aggregate Analysis of Viral Vectors with Novel Wide Pore SEC Columns
From Struggles to Solutions: Strategies for Overcoming Oligonucleotide Challenges
+ VIEW MORE WEBCASTS

Webcasts

What Can CHROMacademy do for You?

Modules

Method Validation - Principles

Modules

Calculations 2 - Basic Result Calculations
Calculations 1 - Units and Unit Conversions
Method Validation - Technique
Quantitation and Calibration
Errors
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Quick Guides

Batch Integration
The What, When, and How of Peak Integration: Part 3. How.
The What, When, and How of Peak Integration: Part 2. When.
The What, When, and How of Peak Integration: Part I. What.

Webcasts

Statistics 101
Chromatographic Method Validation

Webcasts

Top 10 Features of a Successful Chromatography Data System - Part 1
Top 10 Features of a Successful Chromatography Data System - Part 2

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Don't fear the LC-MS - Demystify the process

Practical LC-MS Bootcamp

LC-MS is a complex and unforgiving technique—it often seems like a dark art. However, taking a step back and being shown how to work a system properly can make a huge difference.

*Available with a FREE Lite account

Watch the introduction:

More About The Module

Practical LC-MS

A short, systematic module — unlock the analytical benefits of LC-MS.

Join our experienced technical team as they walk you through the practical set-up and operation on a working LC-MS system. Learn a step-by-step approach and become confident in tackling this form of analysis.

Launch our process-driven Bootcamp module and start practising LC-MS effectively.

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Don't fear the LC-MS - Demystify the process

Practical LC-MS Bootcamp

LC-MS is a complex and unforgiving technique—it often seems like a dark art. However, taking a step back and being shown how to work a system properly can make a huge difference.

Watch the introduction:

More About The Module

Practical LC-MS