LC-MS Introduction
Course Details
LC-MS is a hyphenated technique, which combines the separating power of high performance liquid chromatography (HPLC), with the detection power of mass spectrometry (MS). LC-MS covers a broad range of application areas. This module explores the instrument acquisition methods used and examines the type of data that can be produced from such systems.
Topics include:
- Definitions
- Instrument fundamentals
- LC-MS process
- Why and when to use LC-MS
- Ionization overview
- Atmospheric pressure ionization (API)
- Electrospray ionization (ESI)
- Atomospheric pressure chemical ionization (APCI)
- Atomospheric pressure photoionization (APPI)
- Mass analyzers
- Quadrupole mass analyzer
- Time-of-flight mass analyzer
- Ion trap mass analyzer
- Tandem mass spectrometry (MS/MS)
- Detectors
