Troubleshooting Your HPLC Chromatogram - Selectivity, Resolution, and Baseline Issues

This session examines the common causes of changes in selectivity, loss of chromatographic resolution, and baseline issues – such as splitting, fronting, tailing, and shouldering. Strategies for problem identification and calculation of critical chromatography performance indicators will also be discussed.

Topics include:

• Common causes of problems within your chromatography
• Logical strategies for rapid instrument and separation troubleshooting
• Exploring common problems with:
  • • Mobile phase composition
    • Temperature and pH
    • Column modification and age
    • Sample solvent strength
    • Sample preparation
    • System operating parameters
• Understanding issues with existing problematic methods
• Instigating preventative steps in order to minimize problems in future methods
• Learning how to quickly and accurately identify the common causes of:
  • • Changes in selectivity
    • Loss of chromatographic resolution
    • Baseline issues
• Understanding when and how to employ the correct calculations in order to identify/quantify chromatographic issues
• How these parameters can be affected by:
  • • Mobile phase composition
    • Temperature and pH
    • Column modification and age
    • Sample solvent strength
    • Sample preparation
    • System operation