Troubleshooting Sensitivity Issues (HPLC-UV)

This webcast discusses the impact of sensitivity on HPLC-UV methods. We will discuss what sensitivity is, how we measure it, how it is achieved, and some of the problems that result in issues with sensitivity.

Topics include:

• Limit of detection and quantitation (LOD/LOQ) – including new USP definition
• LOQ – low concentration standards
• Developing methods with high sensitivity
• Benchmarking sensitivity – every method is different
• What causes poor sensitivity – high noise or low signal
• Detector issues – lamp and flow cell issues
• Poor signal 
• High noise