Practical HPLC Troubleshooting Video - Retention Time and Selectivity
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This examples solves an intractable problem of a fast HPLC-MS separation which shows small but disruptive changes in retention time and selectivity for a number of ionizable compounds. The CHROMacademy Troubleshooter is used to diagnose the problem(s) after which we explain how to avoid problems with robustness when using eluent additives which are compatible with mass spectrometric detection.
