HPLC Column Troubleshooting - Can We Fix It?

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This webcast will provide useful practical advice on troubleshooting issues relating to HPLC columns; and how to minimize and resolve these.

Topics include:

  • Columns and fittings
  • Silica based column packing, limitations and batch robustness
  • Measuring column performance – resolution, asymmetry and efficiency
  • Overview of ultra-high performance liquid chromatography – advantages and disadvantages
  • Common column problems
    • High pressure
    • Voids
    • Reversing columns
    • Peak shape issues – what causes tailing and fronting
  • Strategies for minimizing column damage caused by sample matrix
  • Cleaning and regeneration
HPLC Column Troubleshooting - Can We Fix It?