HPLC Column Issues

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The column is at the heart of the HPLC separation, therefore, column related issues can have a big impact on the reliability of our method. We will consider the most common sources of common related issues, allowing you to troubleshoot problematic methods as well as avoiding many of these pitfalls in the first place.

 

Topics include:

  • The HPLC column
  • Effect of physical parameters
    • Length
    • Internal diameter
    • Particle size
    • Solid silica support
  • Bonded phases
  • Endcapping
  • Chemical limitations
  • Column comparison
  • Batch variability
  • Column damage and associated symptoms
  • Column protection and repair
HPLC Column Issues