How to Troubleshoot HPLC Extra Peaks and Contamination Like a Pro

Extra peaks resulting from contamination is our most asked about problem. Contamination can be introduced into the HPLC system at any point of the process, making it an incredibly complex and time-consuming issue to troubleshoot. Previously we have discussed the types and sources of contamination. Now it’s time for us to walk you through a strategy to troubleshoot this problem quickly and effectively using simple experiments to narrow down the source of the problem.

Topics include:

• Types and sources of contamination
• How to clearly define the problem and what it tells us
• Initial experiments to rule out the instrument
• Further investigational experiments