Supercritical Fluid Chromatography
Course Details
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The aim of this module is to introduce supercritical fluid chromatography (SFC) as a powerful solution for chromatographic analysis. The basic aspects of SFC are explained and the fundamental hardware typically used with the technique discussed.
At the end of this module you will be able to understand the benefits and limitations of supercritical fluid chromatography as well as identify some instrumentation typically used in supercritical fluid chromatography.
Topics include:
- Introduction
- Supercritical fluids
- Modes of chromatography
- SFC applications
- SFC instrumentation
- Packed SFC
- Capillary SFC
- The mobile phase
- Exhaust gases
- Organic modifiers
- Pumping issues
- SFC columns
- SFC stationary phases
- SFC column selection
- Detection
- Pressure regulators
- Advantages and disadvantages of SFC
