Mixed-Mode Chromatography – The Answer to Everything?

In this session, our speakers consider the technique of mixed-mode chromatography and the application areas in which this technique can help chromatographers. The mechanisms of mixed-mode chromatography are discussed alongside the parameters which can be used to optimize selectivity and retention. A series of useful illustrative example are presented with strategies for column selection and screening to allow this technique to quickly become part of the analytical armory!

Topics include:

• What is mixed-mode chromatography? - evolution and fundamental retention mechanisms
• Mixed-mode separations – comparison with reversed phase, normal phase, ion exchange, and HILIC chromatography
• Suitable analytes/applications for mixed-mode chromatography
• Column selection
• Retention and selectivity control using organic modifier, buffer strength, and pH
• Screening and initial method development
• Interesting illustrative examples
• The future for mixed-mode phases