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HILIC Conditioning and Equilibration

Course Details Premier

Conditioning and equilibration are important for many HPLC methods to avoid inconsistent retention times between analyses. Particular consideration has to be given to HILIC methods due to the mechanism of analyte retention via an adsorbed water layer at the stationary phase surface. This quick guide will provide you with some practical information on where to start with HILIC column conditioning and equilibration.

HILIC Conditioning and Equilibration