Gradient HPLC - 10 Things You Absolutely Need to Know
Course Details
Premier
Everyone loves a top ten list, so here is our list of “Top Ten Things you Absolutely Need to Know About Gradient HPLC”. Whether you are a novice or seasoned gradient HPLC user, there is something in here for everyone.
Topics include:
- Spot the difference - isocratic vs. gradient elution
- High or low pressure pumps for delivering gradients
- How to optimize essential gradient parameters
- Find the perfect equilibrium with the correct column re-equilibration time
- Don’t dwell on what went wrong, calculate your dwell volume
- It’s a steep learning curve - what effect does gradient steepness have on a separation?
- How to transfer methods - changing flow rate, column, length, column i.d.
- How to avoid (or at least improve) drifting baselines
- Peak capacity - just how many sample components can be separated?
- Which gradient profile will shape your chromatography?
