CHROMtalks - Key Issues with Sample Preparation for Liquid Chromatography

Surveys have shown that errors made during sample preparation procedures are among the largest sources of error in chromatographic separations. For example, in liquid chromatography, lack of attention to sample preparation may introduce particulates into the column, alter the solvent strength of the mobile phase, or render the analytes of interest less detectable. In this presentation, we will look at relationships of solvent selection considerations with peak shapes and injection considerations (solute focusing) and other serious chromatography offenses created by poor sample preparation considerations.