HPLC Chromatographic Parameters - Retention Factor

The aim of any HPLC method is to provide optimum resolution of your analytes in the shortest amount of time. Resolution is governed by three chromatographic parameters, efficiency, selectivity, and retention. This short video will discuss retention factor in more detail, looking at what it describes in terms of your separation and chromatogram, how it can be changed, and what effect it can have on the resolution of a separation.