20 Steps to a Reliable HPLC Mobile Phase

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Selecting a mobile phase and correct preparation are essential for a robust HPLC method. This webcast provides a fail safe 20 step guide to preparing the optimum mobile phase. This includes controlling pH, eluent mixing, filtration and degassing, mobile phase shelf life, and some of the most common mistakes to avoid. 

 

Topics include:

  • pH – correctly controlling pH using buffers
  • Eluent mixing
  • Filtration and degassing
  • Reagent quality
  • Mobile phase shelf life
  • Impact of mobile phase in gradient HPLC
  • System flushing
  • Common mistakes to avoid
20 Steps to a Reliable HPLC Mobile Phase