Critical Evaluation of HPLC Methods

A recent chromacademy webcast looked at the critical evaluation of HPLC methods. This involves examining every facet of the HPLC method, deciding what impact it has on chromatography, and altering these parameters to provide the desired chromatographic results. Our viewers posed some interesting questions during the live Q&A segment of this webcast, so we wanted to share some of them here with you to hopefully encourage some critical evaluation of your own methods.