Tips and Tricks for Carbohydrate Analysis Using HPAE-PAD

High performance anion exchange chromatography (HPAE) combined with pulsed amperometric detection (PAD) is a sensitive analytical technique for native carbohydrates without the need of derivatization. HPAE-PAD can be used to analyze mono-, oligo-, polysaccharides, and glycans. We will present tips and tricks so the system is optimal productive and will provide you the best possible results.

Topics include:

• Eluent preparation and identifying critical points
• Consideration points in the separation conditions
• Column cleaning
• Keeping the electrochemical detection in shape