Peak Purity Algorithms Using Diode Array Detectors

In an ideal chromatographic separation, all sample components would be isolated from each other and detected as fully resolved peaks. However, it is not uncommon to encounter situations when separation is less than ideal, resulting in some degree of overlap. In extreme cases, what appears to be a single peak contains in fact two or more coeluting components. Checking the purity of such peaks is critical in order to correctly interpret the results of the analysis, which is the focus of this guide.