Essential HPLC Protocols

Using the correct start up, shut down, and system column and storage protocols when working with HPLC can be invaluable to ensure that your system functions correctly and that you obtain the desired chromatography. Here are some essential protocols for looking after your HPLC system and column.

Topics include:

• Generic start up protocol
• Generic shut down protocol
• Column and system cleaning/flushing
• Column storage
• Switching between mobile phases