Atmospheric Pressure Chemical Ionization (APCI) Theory

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Atmospheric Pressure Chemical Ionization (APCI) Theory

Atmospheric pressure chemical ionization (APCI) relies on the formation of a plasma of ions comprised, mainly, from the HPLC mobile phase components. The eluent molecules, which are in vast excess compared to the analyte, are ionized by the electron cloud around the corona discharge pin and act as the reagent gas in a chemical ionization process. Analyte molecules are ionized by this reagent gas plasma and are subsequently sampled into the nozzle-skimmer region of the mass spectrometer. This module introduces APCI concepts, explains the function of each component of the APCI interface, and investigates ways in which to optimize and troubleshoot the technique.

Topics include:

Interface overview
Suitable samples for APCI
APCI interfacing details
APCI nebulizer types
APCI nebulizer gas flow
APCI analyte ion declustering
APCI ionization mechanisms
APCI proton affinity
APCI ionization - positive ion mode
APCI ionization - negative ion mode
APCI gas phase reactant ions
APCI reagent gas formation

Atmospheric Pressure Chemical Ionization (APCI) Theory

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