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Tailing peaks create issues with resolution, quantitation (integration), and reproducibility. Peak shape is often the controlling factor when optimizing complex separations, especially when components are present in very different concentrations. Therefore, we are going to look at the top 5 causes of peak tailing provided by our HPLC Troubleshooter – these results have been ranked so are the most likely causes of peak tailing and should, therefore, be your first consideration when you are faced with this problem.

HPLC Troubleshooting Video - Peak Tailing