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Hydrophilic interaction liquid chromatography (HILIC) is a type of chromatography based on a mixed-mode of retention mechanisms. It has found utility in many application areas, but in particular is very applicable to the retention of highly polar molecules which exhibit poor retention characteristics under reversed phase HPLC conditions. This short video will teach you about the primary retention mechanisms and stationary phase interactions involved in HILIC.

HILIC Mechanism and Stationary Phase