HPLC Chromatographic Parameters

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HPLC Chromatographic Parameters

The aim of this module is to introduce and explain the concept of chromatographic resolution. The resolution equation will be defined and its dependence on the chromatographic parameters retention factor, selectivity, and efficiency illustrated. It will be shown how each parameter can be determined and optimized to improve chromatographic resolution.

Upon completion of this module, you will be able to understand and optimize the main chromatographic parameters. You will also be able to explain and demonstrate the concept of peak asymmetry, and understand how to practically improve chromatographic separations.

Topics include:

Resolution
The resolution equation
Retention factor
How to change retention factor
Effect of retention factor on resolution
Selectivity factor
How to change selectivity
Effect of selectivity on resolution
Efficiency
How to change efficiency
Effect of efficiency on resolution
Peak asymmetry

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