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20 Steps to a Reliable Mobile Phase - Q & A

With the mobile phase being integral to the HPLC method it needs to be reliable every time. Which is why there are always lots of questions around mobile phase preparation and design to make sure we get it right first and every time.

Topics include:

  • Amount of mobile phase to prepare
  • Extra peaks
  • Using 100% aqueous
  • Buffers
  • Reagent purity
  • Pump or manual mixing of mobile phases
  • Mobile phase filtration and degassing
  • Mobile phase preparation
  • System flushing
  • Mobile phase lifetime
  • and more!