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Everything You Need to Know About UV Detectors for HPLC

Get more from your UV detector by understanding how it works. The fundamental science behind UV detection and the working principles of this versatile detector will be discussed in detail. Optimization strategies for key parameters will be covered in order to provide you with the tools to correctly set up your detector to provide the optimum performance for your analytical requirements.

 

Topics include:

  • Fundamental UV absorbance theory
  • Understanding the optical arrangements of common UV detectors
  • Data acquisition parameter optimization – bandwidth and slit width settings, acquisition rates, bunch rates, and spectral resolution
  • Detector linearity
  • When and how to choose a reference wavelength to reduce gradient baseline drift and noise
  • Sensitivity – lamp effects, optical and electronic noise, solvent effects etc.
  • Flow cell design
  • Detection robustness - optimizing settings based on chromatographic data and improving your analytical robustness/sensitivity