HPLC Column Chemistry (Thermo)

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The aim of this module is to introduce silica as a major stationary phase support for reversed phase HPLC separations. We explain how bonded phases are produced and highlight the deactivation process used. Modern bonded phases that give an advantage to today’s chromatographer are introduced – including water wettable phases, polar embedded phases, and phases capable of operating at extremes of pH.

Topics include:

Reversed phase stationary phases
Water wettable phases
Aq type stationary phases
Polar embedded phases
Other stationary phase types
Applications of reversed phase HPLC
Selecting reversed phase columns
Applications of normal phase chromatography
Size exclusion chromatography for biomolecules

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