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Analysis at the protein level is common and provides a high level of information such as molecular weight, structural integrity, charge variants, aggregation, and modifications. It is these modifications that we are specifically focusing on when we analyze mAbs at the protein level.

 

Topics include:

  • Protein level analysis introduction
  • Typical protein level conditions
  • Mobile phase additives
  • Particle size and morphology
  • Temperature
  • Flow rate/linear velocity
  • Gradient
  • Mobile phase organic modifier
  • Stationary phase
Reversed Phase - Protein Level Analysis and Optimization