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Hydrophobic interaction chromatography (HIC) is a milder form of reversed phase liquid chromatography (RPLC). Separation of analytes is based on hydrophobic interactions with the stationary phase; therefore, the elution order in HIC enables proteins to be ranked on the basis of their relative hydrophobicity. HIC employs non-denaturing conditions, does not require the use of organic solvents or high temperatures, and separations are carried out at physiological pH allowing the preservation of protein structure. 

 

This quick guide was written by Dr. Dawn Watson (Technical Expert, Crawford Scientific)

Hydrophobic Interaction Chromatography for Biopharmaceutical Analysis