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Choosing the Correct Column and Mobile Phase for LC-MS Peptide Mapping

This webcast will focus on essential parameters for successful peptide mapping by LC-MS. Obtaining complete sequence coverage as well as separation and detection of low-level post-translational modifications is a challenging endeavor. Guidance will be given on matching column, mobile phase, and detector for optimum sequence coverage. Additionally, optimization of key method conditions such as gradient length and temperature will be discussed.

 

Topics include:

  • Choosing the correct column for LC-MS peptide mapping
  • Impact of column chemistry on selectivity and resolution
  • Impact of mobile phases additives on sensitivity, selectivity, and peak capacity