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Reversed Phase - Peptide Mapping LC Conditions and Effects

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Reversed Phase - Peptide Mapping LC Conditions and Effects

This module provides HPLC parameters for carrying out peptide mapping experiments. The effect of each parameter on the resulting chromatography will be detailed.

 

Topics include:

  • Typical peptide mapping conditions
  • Mobile phase additives
  • Temperature
  • Gradient slope/time
  • Column length
  • Particle size and morphology
  • Pore widths
  • Column diameter
Reversed Phase - Peptide Mapping LC Conditions and Effects