Reversed Phase - Peptide Mapping LC Conditions and Effects
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Course Details
This module provides HPLC parameters for carrying out peptide mapping experiments. The effect of each parameter on the resulting chromatography will be detailed.
Topics include:
- Typical peptide mapping conditions
- Mobile phase additives
- Temperature
- Gradient slope/time
- Column length
- Particle size and morphology
- Pore widths
- Column diameter
