Reversed Phase - Introduction and Peptide Level Analysis
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Course Details
Premier
This modules introduces the use of reversed phase HPLC for the characterization of biopharmaceuticals at the peptide level.
Topics include:
- Introduction to reversed phase HPLC
- Analysis at the peptide level
- mAB peptide map
- Common mAb post-translational modifications (PTMs)
- Glycoprofile
- Deamidation
- Oxidation
- Pyroglutamate formation
- Peptide mapping in a routine environment
- Disulfide bridge reduction
- Comparison of digestion enzymes
