N-Glycan Sample Prep and Analysis Workflows for Screening and Characterization of Biotherapeutics
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Course Details
This webcast will discuss next-generation sample preparation workflows for enzymatic release and fluorescent dye labeling of N-glycans from biotherapeutic proteins. Benefits and guidance will be given for the selection of fluorescent dyes and choice of analytical separation and detection method (LC-FLR, LC-MS).
Topics include:
- Choosing a glycan derivatization scheme
- LC-FLD and LC-MS analysis of released N-glycans
- Advice on analytical detection method and fluorescent dye choice
