HILIC for Biomolecules

Hydrophilic interaction liquid chromatography (HILIC) plays an essential role in the characterization of glycosylation of biopharmaceuticals. This module will detail experimental conditions for HILIC analyses in relation to biomolecules.

Topics include:

• Introduction to HILIC for biomolecules
• Glycosylation sites and glycans
• Glycan mapping workflow
• Typical glycan mapping HILIC conditions
• Method precision and accuracy
• Resolving power for various protein biopharmaceuticals
• Glycan analysis during biosimilar development