CHROMtalks - Tips and Tricks for Contemporary LC Separations of Biomolecules

This presentation will highlight some challenges commonly observed when characterizing protein biopharmaceutical samples (such as monoclonal antibodies and related compounds) in non-denaturing liquid chromatography (LC) and how to mitigate them. The focus will be on two different LC modes, namely ion-exchange chromatography (IEX) and size-exclusion chromatography (SEC), which are widely used for charge variant and size variant separations. Helpful information will be shared during the talk to help achieve successful IEX and SEC separations.