Technique

Reversed Phase - Introduction and Peptide Level Analysis

This modules introduces the use of reversed phase HPLC for the characterization of biopharmaceuticals at the peptide level.

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Micro Elution Solid Phase Extraction in BioAnalysis – Improving Bioanalytical Performance

This webcast presents the features and benefits of using micro elution SPE formats in order to improve the sample preparation process and drive down sample volumes.

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Techniques Employed in Biopharmaceutical Analysis – Part I Reversed Phase and HILIC

This webcast explores the use of reversed phase and HILIC chromatographic techniques in the analysis of biopharmaceuticals.

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Techniques Employed in Biopharmaceutical Analysis – Part II Ion Exchange and SEC

This webcast explores the use of ion exchange, size exclusion, and hydrophobic interaction chromatography for the analysis of biopharmaceuticals.

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HPLC Techniques in Biopharmaceutical Analysis

This webcast introduces the wide array of analytical techniques that are required to fully characterize biopharmaceuticals.

Hydrophobic Interaction Chromatography of mAbs and ADCs

This webcast details the important factors that need to be considered to develop robust and reliable UHPLC-based HIC separations and how novel HIC stationary phases can solve current industry challenges in determining critical quality attributes of mAbs and ADCs.

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UV or MS, Derivatized or Not? Choosing & Optimizing Workflows for Amino Acid Analysis in Spent Media

This webcast will discuss workflow options for spent media analysis, focusing on LC/UV and LC/MS methods.

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Native Mode Analysis - Intact Workflow Solutions for Characterizing Biomolecules by HIC and SEC

In this webcast, we will demonstrate the utility of SEC and HIC methods for intact analysis of antibodies and ADCs.

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Applying High Resolution and High Efficiency SEC Technology to Biotherapeutics

This webcast will discuss the superior performance in high resolution, high efficiency, and high throughput analysis of mAbs, ADCs and fusion proteins that can be achieved with SEC columns with optimized sub-2 µm particle and unique hydrophilic bonding chemistry.

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Advances in Full Characterization of Protein Biopharmaceutical Post-Translational Modifications

This webcast will consider the types of post translational modifications which can be assessed and what chromatographic or mass spectrometric methods should be used to identify them.

Hydrophobic Interaction Chromatography for Biopharmaceutical Analysis

This quick guide details the use of hydrophobic interaction chromatography (HIC) for the analysis of biopharmaceuticals.

HILIC, IEX, and SEC for the Analysis of Biomolecules

This quick guide focusses on the use of HILIC, IEX, and SEC for the characterization of biomolecules.

Reversed Phase HPLC for the Analysis of Biomolecules

The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in their analysis.

Analysis of Next Generation Protein Biopharmaceuticals

This webcast will focus on the chromatographic and mass spectrometric analysis of next generation biopharmaceuticals, for example, biosimilars, biobetters, and antibody drug conjugates.

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Introduction to Two-Dimensional Liquid Chromatography for Biomolecule Separations

This webcast will provide an overview of the key concepts required for successful 2D-LC method development, and several examples of recently developed 2D-LC separations of biomolecules.

Identification, Mapping, and Relative Quantitation of SARS-CoV-2 Spike Glycopeptides by Mass-Retention

This webcast will discuss the technical issues surrounding the characterization of SARS–CoV–2 spike (S) protein glycosylation and its importance for quality control of expressed spike protein.

Mass Spectrometry at the Peptide Level

This module will allow you to understand how to utilize mass spectrometry based peptide mapping, including how to digest samples, where to start with LC-MS conditions, analyze the data produced, and how to use MS/MS experiments to further enhance confidence in peptide identity, sequence, modifications, and modification sites.

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Mass Spectrometry at the Glycan Level

This module will introduce the use of mass spectrometry for glycan analysis.

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Reversed Phase - Peptide Mapping LC Conditions and Effects

This module provides HPLC parameters for carrying out peptide mapping experiments. The effect of each parameter on the resulting chromatography will be detailed.

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Affinity Chromatography Techniques

This module introduces the principles of affinity chromatography for the analysis of biomolecules.

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Mass Spectrometry at the Protein Level

his module will explore the different mass spectrometric techniques used for protein level analysis and look at the information provided by each technique.

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Mass Spectrometry Basics for Biomolecule Analysis

This module covers the fundamentals of mass spectrometry for the analysis of biomolecules.

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Ion Exchange Techniques

This module discusses ion exchange techniques and applications for the analysis of biomolecules.

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Size Exclusion Techniques

This module focuses on size exclusion chromatography for the analysis of biomolecules.

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HILIC for Biomolecules

This module will detail experimental conditions for HILIC analyses in relation to biomolecules.

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Reversed Phase - Protein Level Analysis and Optimization

This module discusses the analysis of proteins using reversed phase chromatography.

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Introduction to Peptide Analysis by HPLC

This webcast will introduce the process by which the parameters of reversed phase HPLC can be optimized to analyze peptides; we will consider the stationary phase chemistry which impacts selectivity, as well as the impact the physical attributes of the column have on peptide separations.

First Steps in Characterizing Biotherapeutics

This quick guide will take a practical look at the three most common chromatographic techniques used for analyzing biotherapeutics, size exclusion (SEC), ion exchange (IEX), and reversed phase (RP) chromatographies.

High-throughput and High-resolution N-glycan Profiling Workflows Using InstantPC

This webcast will describe complete N-glycan quantitation workflows using the Agilent AdvanceBio InstantPC label for high-throughput and high-resolution glycan profiling using hydrophilic interaction liquid chromatography (HILIC) with detection by fluorescence (FLD) or mass spectrometry (MS).

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In-depth Characterization of Biopharmaceuticals by Multidimensional LC-MS

This webcast will highlight the power of 2D-, 3D- and 4D-LC in hyphenation to mass spectrometry (MS) for the detailed characterization of protein biopharmaceuticals.

Introduction to Ion Mobility Spectrometry (IMS)

This introductory module will detail IMS instrument components and how separation occurs as well as considering the advantages of hyphenating the technique with chromatography and particularly mass spectrometry to provide two-dimensional separation data which can be particularly useful in the analysis of biomolecules.  

CHROMtalks - Tips and Tricks for Contemporary LC Separations of Biomolecules

This presentation will highlight some challenges commonly observed when characterizing protein biopharmaceutical samples (such as monoclonal antibodies and related compounds) in non-denaturing liquid chromatography (LC) and how to mitigate them.

LC/MS MAM Analysis of Adeno-Associated Virus Particles

This webcast will discuss several workflows for characterizing AAV CQAs, including product identity, post-translational modifications, and host cell proteins. 

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HPLC Analysis of Oligonucleotides

In this webcast we present an overview of the HPLC analysis of oligonucleotides. We’ll provide the basics on nucleotides and how synthetic oligonucleotides are produced, introducing you to some of the language used in support of these molecules.

N-Glycan Sample Prep and Analysis Workflows for Screening and Characterization of Biotherapeutics

This webcast will discuss next-generation sample preparation workflows for enzymatic release and fluorescent dye labeling of N-glycans from biotherapeutic proteins.

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The Power of LC-MS in the Characterization of Protein Biopharmaceuticals

This webcast will highlight the power of liquid chromatography combined with mass spectrometry in the characterization of protein biopharmaceuticals.

The Analysis of Post-Translational Modifications Using LC-MS/MS

In this webcast the principles behind the analysis of a number of key post-translational modifications (PTMs) using LC-MS/MS techniques will be explained.

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Native and Denaturing Mode Size Exclusion Analysis of Biotherapeutics

In this webcast, we will present SEC characterization of various biotherapeutic proteins.

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Introduction to Mass Spectrometry for Biomolecules

This module provides an introduction to mass spectrometry for biomolecules.

Fundamentals of Biomolecules

This quick guide covers the fundamentals of what biomolecules are, how are they made naturally, how to make them synthetically using biotechnology, and which analytical techniques are key to characterizing them (including some tips on where to start with initial analyses).