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Choosing the Correct Column and Mobile Phase for LC-MS Characterization of Biologics

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Choosing the Correct Column and Mobile Phase for LC-MS Characterization of Biologics

This webcast will focus on the parameters which are essential for the development of a successful RPLC-MS method for the characterization of a monoclonal antibody. The methods will allow for the analysis of the intact species, determination of absolute mass, examination of the major fragments, (Fab/Fc and Hc/Lc), and investigation of the small peptides produced by protease digestion. The importance of pore size and mobile phase additives will be addressed and guidance given on how to optimize methods for sensitivity and resolution.

 

Topics include:

  • Choosing the correct column pore size for mAb intact, fragment, and peptide analysis
  • Impact of column chemistry on selectivity and resolution
  • Impact of mobile phases additives on selectivity and sensitivity
Choosing the Correct Column and Mobile Phase for LC-MS Characterization of Biologics