Mass Analyzers Used in Biomolecule Analysis

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Mass Analyzers Used in Biomolecule Analysis
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The working principles of time-of-flight (ToF), orbitrap, and fourier transform ion cyclotron resonance (FT-ICR) mass analyzer will be discussed in this module.

 

Topics include:

  • Time-of-flight (ToF) mass analyzers
  • Time-of-flight (Tof) resolution
  • ToF - the reflectron
  • ToF - increased resolution using reflectrons
  • ToF - performance limitations
  • Orthogonally accelerated ToF instruments
  • oaToF pusher electrode
  • oaToF pusher pulse rate
  • oaToF deflecting voltage
  • oaToF interfacing details
  • oaToF mass resolution and accuracy
  • oaToF dynamic range
  • Orbitrap overview and anatomy
  • Orbitrap - continuous ion source
  • Orbitrap - ion injection
  • Orbitrap - Fourier transform mode
  • Orbitrap - mass selective instability (MSI) mode
  • Orbitrap - multiply charged ions and calibration
  • Orbitrap - high molecular weight application
  • Orbitrap - low molecular weight application
  • Orbitrap - comparison with other analyzers
  • Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)
Mass Analyzers Used in Biomolecule Analysis