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The CHROMacademy Essential Guide Webcast:
Understanding and Improving SPE


Thursday 16th October 2014,
8:00am PDT / 11:00am EDT / 16:00 BST / 17:00 CEST

Solid phase extraction (SPE) is one of the most effective and widely used sample preparation techniques for liquid and solid samples. As analysts are asked to detect analytes at ever decreasing levels, well performed sample preparation is a must. This Essential Guide webcast will help you to better understand how SPE works, the stationary phase chemistries which are available and which analytes they are applicable too, and how to avoid common pitfalls in order to improve your SPE method.

  • Understanding SPE mechanisms
  • Optimizing selectivity through sorbent selection
  • Properly equilibrating a sorbent
  • Investigating low recovery
  • The importance of soak and drying steps
  • How to choose the correct bed mass
  • Assessing breakthrough
  • Optimizing wash solvent strength and effectiveness
  • Optimizing elution solvent strength and volume
  • Polymeric sorbents, molecularly imprinted polymers, and random access media for SPE

Key Learning Objectives:

  • Understand the fundamental chemistry behind SPE
  • Learn how to select the correct SPE method for your analytes
  • Gain practical tips for improving SPE

Who Should Attend:

  • Anyone working with GC
  • Anyone working with HPLC
  • Anyone working with mass spec
  • Anyone with dirty samples
  • Anyone who wants to avoid poor or inconsistent quantitative results
 
Understanding and Improving SPE
 

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ThermoUnderstanding and Improving SPE Tutorial

Solid phase extraction (SPE) is one of the most effective and widely used sample preparation techniques for liquid and solid samples. As analysts are asked to detect analytes at ever decreasing levels, well performed sample preparation is a must. This Essential Guide webcast will help you to better understand how SPE works, the stationary phase chemistries which are available and which analytes they are applicable too, and how to avoid common pitfalls in order to improve your SPE method.

Watch the webcast and delve into our CHROMacademy material to further your knowledge of SPE.

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eLearning Modules
 
SPE Overview

The perfect quick guide to solid phase extraction (SPE). Understand the essentials of SPE including, the terminology, sorbent characteristics, and protocol steps.

SPE Terminology | SPE Sorbent Substrates | Choosing Sorbent Mass | Protocol Steps in SPE

 
SPE Mechanisms

A comprehensive guide to solid phase extraction (SPE) which covers the different modes of SPE (i.e. polar, non-polar, ion exchange etc.), as well as, discussing each of the protocol steps involved in an SPE extraction. This elearning module will furnish you with the knowledge required to choose the correct SPE mode, phase, and conditions resulting in highly efficient analyte extraction procedures.

Non-Polar Sample Pretreatment | Equilibration and Sample Loading | Polar Sample Pretreatment |
Polar Sorbent Conditioning, Equilibration and Sample Loading
| Cation-Exchange Sample Pretreatment |
Sorbent Conditioning, Equilibration and Sample Loading

 
Method Development

This e-Learning module investigates various aspects of practical SPE including; sorbent column processing, sorbent bed geometry, the use of disc based SPE, and the impact of fines on SPE. The basic principles and stages of SPE method development will be discussed along with an overview of troubleshooting SPE protocols.

Sorbent Bed Geometry | SPE Method Development - Overview | Mechanism Selection 1 | Soak Steps | Drying Steps | Elution Optimization | Recovery Problems 1

 
Quick Guides
 
SPE Method Development Interactive Learning Tool

This tool gives a simple and structured approach to developing a reversed phase Solid Phase Extraction (SPE) method. The interactive learning tool allows for the evaluation of different sets of conditions and demonstrates what effect they have on the resulting chromatogram.

SPE Method Development Tool

 
What Can You Learn in 5 Minutes - SPE?

This clear and concise 5 minute guide will explain the fundamental concepts of SPE and how to avoid common errors.

SPE sample preparation

 
Choosing the Correct Sample Preparation Technique

The correct choice of sample preparation method for a sample prior to analysis can have a myriad of benefits; properly prepared samples will produce simpler chromatograms free from interfering peaks, instrument parts will be protected from harmful contaminants which could result in break downs and the need for increased maintenance, and the sample will be compatible with the analysis method (correct pH, solvent etc.) minimizing deleterious chromatographic effects.

Sample Preparation Technique

 
Understanding the Mechanisms of Solid Phase Extraction

7 Key Secrets to Understanding Solid Phase Extraction. This webcast and tutorial provide a highly concentrated primer that will help you to understand all of the key stages in SPE protocol development. This understanding is crucial for improving your SPE technique and ultimately to develop better solid phase extraction methods.

Mechanisms of Solid Phase Extraction

 
 
 
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  • Understanding SPE mechanisms
  • Optimizing selectivity through sorbent selection
  • Properly equilibrating a sorbent
  • Investigating low recovery
  • The importance of soak and drying steps
  • How to choose the correct bed mass
  • Assessing breakthrough
  • Optimizing wash solvent strength and effectiveness
  • Optimizing elution solvent strength and volume
  • Polymeric sorbents, molecularly imprinted polymers, and random access media for SPE

Who Should Attend:

  • Anyone working with GC
  • Anyone working with HPLC
  • Anyone working with mass spec
  • Anyone with dirty samples
  • Anyone who wants to avoid poor or inconsistent quantitative results

Dr. Douglas Raynie
Associate Research Professor,
Department of Chemistry and Biochemistry, South Dakota State University

Dr Dawn Watson
Technical Specialist
Crawford Scientific