Column Chemistry II, Eluent Design and Sample Screening
In our second session we conclude our discussions on HPLC column selection, including a discussion on stationary phase characterisation and sources of information to aid column selection.
We will also consider column geometry and the selection of column dimensions to suit our analytical objectives. Following this, we will begin our discussions on logical method development, including choice of the separation mode and initial sample screening using scouting gradients and we will begin our discussion on eluent design and optimisation by investigating the various solvents available and their characteristics.
Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.
Tony Taylor B.Sc. CChem FRSC Cert. Ed.
Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.
Associated Training Material from CHROMacademy
FAQs will be added as students progress through the course.