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HPLC Method Development Video Training Course
Session 1

 
 
Setting Method Objectives, Sample Preparation & Column Chemistry I

We begin by discussing how to set analytical objectives, because, in the words of the Wizard of Oz, if you don’t know where you are going, how do you know when you’ve arrived! This will involve assessment of the sample matrix and analyte chemistry (where known) and their influence on designing methods and setting analytical objectives.

We’ll look at the sample preparation options available to us and the types of preparation that might be required for various application types and detection techniques. We also begin our discussion on selecting the correct mode of chromatography for our separation and examine some of the stationary phase chemistries and morphologies available to today’s method developers.

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Tutor support

Amy Claydon BSc PhD

Amy began using chromatography at the start of her academic career with nanoflow-liquid chromatography-mass spectrometry, and a variety of other proteomics techniques, for her research into the dynamic proteome. The laboratory also focused on gas chromatographic separations of volatile biological samples.
After using chromatography for 10 years, Amy has now moved into a more training-based role with Crawford Scientific, where she hopes to share the knowledge she gained during her time in the laboratory.

  Amy Claydon

Tony Taylor B.Sc. CChem FRSC Cert. Ed.

Tony has been doing, researching, teaching and training in analytical chemistry for the past 28 years. He comes from a pharmaceutical and polymer analysis background and continues to work with both liquid and gas phase techniques at Crawford Scientific (UK).
His main interests are the use of LC-MS and GC-MS for structural characterisation and the quantitation of trace components in complex matrices. He is professionally qualified trainer and is Technical Director of the CHROMacademy.

  Tony Taylor

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Associated Training Material from CHROMacademy

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FAQs

FAQs will be added as students progress through the course.

 
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